Quantitative single-molecule microscopy reveals that CENP-A deposition occurs during G2 in fission yeast
نویسندگان
چکیده
David Lando1,5,†, Ulrike Endesfelder3,†, Harald Berger4, Lakxmi Subramanian4, Paul D. Dunne2, James McColl2, David Klenerman3, Antony M. Carr5, Markus Sauer3, Robin C. Allshire4, Mike Heilemann3 and Ernest D. Laue1 Department of Biochemistry, and Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK Department of Biotechnology and Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Wurzburg, Germany Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK
منابع مشابه
Quantitative single-molecule microscopy reveals that CENP-ACnp1 deposition occurs during G2 in fission yeast
The inheritance of the histone H3 variant CENP-A in nucleosomes at centromeres following DNA replication is mediated by an epigenetic mechanism. To understand the process of epigenetic inheritance, or propagation of histones and histone variants, as nucleosomes are disassembled and reassembled in living eukaryotic cells, we have explored the feasibility of exploiting photo-activated localizatio...
متن کاملBiphasic incorporation of centromeric histone CENP-A in fission yeast.
CENP-A is a centromere-specific histone H3 variant that is essential for kinetochore formation. Here, we report that the fission yeast Schizosaccharomyces pombe has at least two distinct CENP-A deposition phases across the cell cycle: S and G2. The S phase deposition requires Ams2 GATA factor, which promotes histone gene activation. In Delta ams2, CENP-A fails to retain during S, but it reaccum...
متن کاملA NASP (N1/N2)-Related Protein, Sim3, Binds CENP-A and Is Required for Its Deposition at Fission Yeast Centromeres
A defining feature of centromeres is the presence of the histone H3 variant CENP-A(Cnp1). It is not known how CENP-A(Cnp1) is specifically delivered to, and assembled into, centromeric chromatin. Through a screen for factors involved in kinetochore integrity in fission yeast, we identified Sim3. Sim3 is homologous to known histone binding proteins NASP(Human) and N1/N2(Xenopus) and aligns with ...
متن کاملEctopic centromere nucleation by CENP--a in fission yeast.
The centromere is a specific chromosomal locus that organizes the assembly of the kinetochore. It plays a fundamental role in accurate chromosome segregation. In most eukaryotic organisms, each chromosome contains a single centromere the position and function of which are epigenetically specified. Occasionally, centromeres form at ectopic loci, which can be detrimental to the cell. However, the...
متن کاملTelomeric Repeats Facilitate CENP-ACnp1 Incorporation via Telomere Binding Proteins
The histone H3 variant, CENP-A, is normally assembled upon canonical centromeric sequences, but there is no apparent obligate coupling of sequence and assembly, suggesting that centromere location can be epigenetically determined. To explore the tolerances and constraints on CENP-A deposition we investigated whether certain locations are favoured when additional CENP-A(Cnp1) is present in fissi...
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تاریخ انتشار 2012